Hotspot

rpoA

LSC · 80784-81797 · 1014 bp

Back to Trichocladus

Pi 0.000986

MarkerSeek score 47.30

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1014
variable_sites: 1
pi: 0.000986
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	CATTTCGTCGTCCAGTAGCA	TTGTACGGGATGTAACCCCT	50.0	50.0	58.0	58.0	1462-1467 bp	1.0	80.2
rpoA_p2	CCTTCGGGGGATGGATCTAT	TTGTACGGGATGTAACCCCT	55.0	50.0	58.0	58.0	1861-1866 bp	1.0	79.8
rpoA_p3	CATTTCGTCGTCCAGTAGCA	AAGAGGGACACCATTTGCTG	50.0	50.0	58.0	58.1	1620-1625 bp	1.0	79.8
rpoA_p4	CATTTCGTCGTCCAGTAGCA	AAAATAAGGGCCTCTGCTCG	50.0	50.0	58.0	58.0	2040 bp	0.5	59.6
rpoA_p5	CCTTCGGGGGATGGATCTAT	AAAATAAGGGCCTCTGCTCG	55.0	50.0	58.0	58.0	2439 bp	0.5	59.3