Hotspot

petL

LSC · 68591-68698 · 108 bp

Back to Trichocladus

Pi 0.436

MarkerSeek score 66.40

Primer available yes

Label petL

Feature Metadata

label_name: petL
feature_type: gene
region: LSC
length_bp: 108
variable_sites: 24
pi: 0.436
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.51
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL_p1	ATGTGAATCCGACGAGTTGG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	1203-1208 bp	1.0	91.8
petL_p2	GCCGCTAGTAGAAAACCGAA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	998 bp	0.5	59.9
petL_p3	AAGCCGCTAGTAGAAAACCG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	1000 bp	0.5	59.8
petL_p4	GCCGCTAGTAGAAAACCGAA	TGATCACCACGCCTGTATTG	50.0	50.0	58.0	58.0	782 bp	0.5	59.8
petL_p5	GCCGCTAGTAGAAAACCGAA	GATCACCACGCCTGTATTGT	50.0	50.0	58.0	58.0	781 bp	0.5	59.8