Hotspot

petB

LSC · 77770-79209 · 1440 bp

Back to Trichocladus

Pi 0.00347

MarkerSeek score 60.70

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1440
variable_sites: 5
pi: 0.00347
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AGAACCACGAAGTCCTAGCT	ATTGTTCCTCCCCCAAATGA	50.0	45.0	58.1	56.7	1605 bp	1.0	69.1
petB_p2	AGAACCACGAAGTCCTAGCT	TTGTTCCTCCCCCAAATGAT	50.0	45.0	58.1	56.7	1604 bp	1.0	69.1
petB_p3	TCACAAGAACCACGAAGTCC	ATTGTTCCTCCCCCAAATGA	50.0	45.0	57.8	56.7	1610 bp	1.0	67.8
petB_p4	TCACAAGAACCACGAAGTCC	TTGTTCCTCCCCCAAATGAT	50.0	45.0	57.8	56.7	1609 bp	1.0	67.8
petB_p5	CACAAGAACCACGAAGTCCT	TTGTTCCTCCCCCAAATGAT	50.0	45.0	57.8	56.7	1608 bp	1.0	67.8