Hotspot

ndhD

SSC · 119516-121045 · 1530 bp

Back to Trichocladus

Pi 0.00261

MarkerSeek score 47.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 4
pi: 0.00261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GTAATCCGGCGAGAACCATT	AGAGGACTGTGTCGGTTGTA	50.0	50.0	58.1	58.0	1931-1933 bp	1.0	80.4
ndhD_p2	GTAATCCGGCGAGAACCATT	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.1	58.0	1945-1947 bp	1.0	80.4
ndhD_p3	AGGTGCAAATTCCGCAATTG	AGAGGACTGTGTCGGTTGTA	45.0	50.0	57.9	58.0	2115-2117 bp	1.0	80.1
ndhD_p4	CAAGGTGCAAATTCCGCAAT	AGAGGACTGTGTCGGTTGTA	45.0	50.0	57.9	58.0	2117-2119 bp	1.0	80.1
ndhD_p5	AGGTGCAAATTCCGCAATTG	TCTGCTCCAAGAACAGAGGA	45.0	50.0	57.9	58.0	2129-2131 bp	1.0	80.0