Hotspot

ccsA-ndhD

SSC · 119271-119515 · 245 bp

Back to Trichocladus

Pi 0.0449

MarkerSeek score 67.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 245
variable_sites: 11
pi: 0.0449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCAAATTCCGCAATTGTAGCT	TCAGTTGACAAGGTCGAAACT	42.9	42.9	58.1	57.5	413-416 bp	1.0	60.9
ccsA-ndhD_p2	GCAAATTCCGCAATTGTAGC	TCAGTTGACAAGGTCGAAACT	45.0	42.9	56.6	57.5	413-416 bp	1.0	59.0
ccsA-ndhD_p3	GCAATTGTAGCTTCTATGGGC	TCAGTTGACAAGGTCGAAACT	47.6	42.9	57.4	57.5	404-407 bp	1.0	56.8
ccsA-ndhD_p4	GCAAATTCCGCAATTGTAGCT	TCAGTTGACAAGGTCGAAAC	42.9	45.0	58.1	55.9	413-416 bp	1.0	56.7
ccsA-ndhD_p5	GCAAATTCCGCAATTGTAGCT	CAGTTGACAAGGTCGAAACT	42.9	45.0	58.1	55.9	412-415 bp	1.0	56.6