Hotspot

rpl16-rps3

LSC · 85472-85616 · 145 bp

Back to Trichocladus

Pi 0.0138

MarkerSeek score 60.40

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 145
variable_sites: 2
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AGAACCAACGAGTCACACAC	CCCTACAAACCATTCGAGCT	50.0	50.0	58.1	57.6	276 bp	1.0	76.8
rpl16-rps3_p2	GAACCAACGAGTCACACACT	CCCTACAAACCATTCGAGCT	50.0	50.0	58.1	57.6	275 bp	1.0	76.8
rpl16-rps3_p3	AGAACCAACGAGTCACACAC	GGGTTCCCCTACAAACCATT	50.0	50.0	58.1	57.4	282 bp	1.0	75.1
rpl16-rps3_p4	GAACCAACGAGTCACACACT	GGGTTCCCCTACAAACCATT	50.0	50.0	58.1	57.4	281 bp	1.0	75.1
rpl16-rps3_p5	GAACCAACGAGTCACACACT	CCCCTACAAACCATTCGAGC	50.0	55.0	58.1	58.6	276 bp	1.0	75.1