Hotspot

petL-petG

LSC · 68699-68920 · 222 bp

Back to Trichocladus

Pi 0.106

MarkerSeek score 75.50

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 222
variable_sites: 21
pi: 0.106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	ATGTGAATCCGACGAGTTGG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	1203-1208 bp	1.0	91.8
petL-petG_p2	GCCGCTAGTAGAAAACCGAA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	998 bp	0.5	59.9
petL-petG_p3	AAGCCGCTAGTAGAAAACCG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	1000 bp	0.5	59.8
petL-petG_p4	GCCGCTAGTAGAAAACCGAA	TGATCACCACGCCTGTATTG	50.0	50.0	58.0	58.0	782 bp	0.5	59.8
petL-petG_p5	GCCGCTAGTAGAAAACCGAA	GATCACCACGCCTGTATTGT	50.0	50.0	58.0	58.0	781 bp	0.5	59.8