Hotspot

trnT-GGU-psbD

LSC · 33673-35059 · 1387 bp

Back to Trichocladus

Pi 0.00217

MarkerSeek score 60.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1387
variable_sites: 3
pi: 0.00217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1551-1558 bp	1.0	77.5
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	GACCGGACCAACCTACAAAA	50.0	50.0	57.9	57.7	1565-1572 bp	1.0	77.4
trnT-GGU-psbD_p3	GCCCTTTTAACTCAGCGGTA	TAGACCGGACCAACCTACAA	50.0	50.0	57.9	57.7	1567-1574 bp	1.0	77.0
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	CCCTTCGTAACCAGTCATCC	50.0	55.0	57.9	57.7	1535-1542 bp	1.0	76.9
trnT-GGU-psbD_p5	GCCCTTTTAACTCAGCGGTA	GAGCAATAGACCGGACCAAC	50.0	55.0	57.9	58.3	1573-1580 bp	1.0	76.7