Hotspot

petN-psbM

LSC · 29528-30706 · 1179 bp

Back to Trichocladus

Pi 0.00509

MarkerSeek score 61.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1179
variable_sites: 6
pi: 0.00509
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	GCTACGGCACTGTTCATTCT	50.0	50.0	58.2	58.3	1340-1343 bp	1.0	77.0
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	GCTACGGCACTGTTCATTCT	50.0	50.0	57.6	58.3	1279-1282 bp	1.0	75.4
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TGCTACGGCACTGTTCATTC	50.0	50.0	58.2	58.6	1341-1344 bp	1.0	74.7
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACGGCACTGTTCATTC	50.0	50.0	57.6	58.6	1280-1283 bp	1.0	73.1
petN-psbM_p5	ATAGTAAGTCTCGCTTGGGC	GCTACGGCACTGTTCATTCT	50.0	50.0	57.1	58.3	1341-1344 bp	1.0	71.0