Hotspot

trnS-GCU-trnS-CGA

LSC · 8338-9233 · 896 bp

Back to Trichocladus

Pi 0.00563

MarkerSeek score 62.80

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 896
variable_sites: 5
pi: 0.00563
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	CAATCCGACGCTTTAGTCCA	GAATTAAACCGAGGGACCCC	50.0	55.0	58.0	58.0	1002-1009 bp	1.0	80.6
trnS-GCU-trnS-CGA_p2	AATCCGACGCTTTAGTCCAC	GAATTAAACCGAGGGACCCC	50.0	55.0	58.0	58.0	1001-1008 bp	1.0	80.6
trnS-GCU-trnS-CGA_p3	ACGGAAAGAGAGGGATTCGA	GAATTAAACCGAGGGACCCC	50.0	55.0	58.2	58.0	1056-1063 bp	1.0	79.4
trnS-GCU-trnS-CGA_p4	TGGAAACGGAAAGAGAGGGA	GAATTAAACCGAGGGACCCC	50.0	55.0	58.3	58.0	1061-1068 bp	1.0	78.4
trnS-GCU-trnS-CGA_p5	AGCAATCCGACGCTTTAGTC	GAATTAAACCGAGGGACCCC	50.0	55.0	58.4	58.0	1004-1011 bp	1.0	77.8