Hotspot

trnK-UUU-rps16

LSC · 4349-5225 · 877 bp

Back to Trichocladus

Pi 0.00685

MarkerSeek score 54.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 877
variable_sites: 6
pi: 0.00685
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGATGGTATGGACGAATCC	AGAAGGCGGAGGTTTTTACG	55.0	50.0	57.9	58.2	1025-1031 bp	1.0	78.3
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	AGAAGGCGGAGGTTTTTACG	50.0	50.0	58.2	58.2	964-970 bp	1.0	77.7
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	AGAAGGCGGAGGTTTTTACG	50.0	50.0	58.2	58.2	963-969 bp	1.0	77.7
trnK-UUU-rps16_p4	CCGATGGTATGGACGAATCC	GGTTTTTACGGAACTTCGCC	55.0	50.0	57.9	57.7	1015-1021 bp	1.0	77.3
trnK-UUU-rps16_p5	CCGATGGTATGGACGAATCC	TCAAAGAAGGCGGAGGTTTT	55.0	45.0	57.9	57.6	1029-1035 bp	1.0	77.0