Hotspot

rpl14-rpl16

LSC · 83539-83688 · 150 bp

Back to Trema

Pi 0.0311
MarkerSeek score 59.50
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
150
variable_sites
12
pi
0.0311
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 TTTCTGGCTCCGCTGTTATC GGGTTCGGGAAAAGGATCTC 50.0 55.0 58.0 58.0 340-374 bp 1.0 86.6
rpl14-rpl16_p2 TTTCTGGCTCCGCTGTTATC GGTTCGGGAAAAGGATCTCC 50.0 55.0 58.0 58.0 339-373 bp 1.0 86.6
rpl14-rpl16_p3 TTTCTGGCTCCGCTGTTATC GTTCGGGAAAAGGATCTCCC 50.0 55.0 58.0 58.0 338-372 bp 1.0 86.6
rpl14-rpl16_p4 TTTCTGGCTCCGCTGTTATC CCCGATTCCGTAAACACCAT 50.0 50.0 58.0 58.0 569-603 bp 1.0 85.7
rpl14-rpl16_p5 TGCGCCAAAAATTCGAGTTC CCCGATTCCGTAAACACCAT 45.0 50.0 58.0 58.0 819-853 bp 1.0 84.0