Hotspot

trnT-UGU-trnL-UAA

LSC · 48480-49372 · 893 bp

Back to Trema

Pi 0.0125
MarkerSeek score 67.30
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
893
variable_sites
25
pi
0.0125
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 923-959 bp 1.0 83.0
trnT-UGU-trnL-UAA_p2 GAGTCGAACCGATGACCATC CCGTAGCGTCTACCGATTTC 55.0 55.0 58.2 58.2 954-990 bp 1.0 82.6
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 917-953 bp 1.0 81.8
trnT-UGU-trnL-UAA_p4 TGGAGAAAAGCCAGCTATCG CCGTAGCGTCTACCGATTTC 50.0 55.0 57.7 58.2 974-1010 bp 1.0 81.3
trnT-UGU-trnL-UAA_p5 AAAAGCCAGCTATCGGAGTC CCGTAGCGTCTACCGATTTC 50.0 55.0 57.7 58.2 969-1005 bp 1.0 81.3