Hotspot

atpA-atpF

LSC · 12044-12114 · 71 bp

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Pi 0.0394
MarkerSeek score 72.80
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
71
variable_sites
6
pi
0.0394
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 TTTCATCGGCTCGAATGGTT ACAAGGAGCTCTAGGAACTCT 45.0 47.6 57.9 57.5 203-207 bp 1.0 70.9
atpA-atpF_p2 TTTCATCGGCTCGAATGGTT AGGAGCTCTAGGAACTCTGA 45.0 50.0 57.9 56.5 200-204 bp 1.0 70.6
atpA-atpF_p3 TCATCGGCTCGAATGGTTAC ACAAGGAGCTCTAGGAACTCT 50.0 47.6 57.8 57.5 201-205 bp 1.0 70.1
atpA-atpF_p4 TCATCGGCTCGAATGGTTAC AGGAGCTCTAGGAACTCTGA 50.0 50.0 57.8 56.5 198-202 bp 1.0 69.7
atpA-atpF_p5 TTGTTCAATACGTTCGCGGA ACAAGGAGCTCTAGGAACTCT 45.0 47.6 58.2 57.5 235-239 bp 1.0 69.5