Hotspot

psbI-trnS-GCU

LSC · 8214-8435 · 222 bp

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Pi 0.0299
MarkerSeek score 59.30
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
222
variable_sites
13
pi
0.0299
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 GCGTAATCCTGGACGTGAAG TGGACTAAAGCGTCGGATTG 55.0 50.0 58.7 58.0 309-364 bp 1.0 80.1
psbI-trnS-GCU_p2 GCGTAATCCTGGACGTGAAG GTGGACTAAAGCGTCGGATT 55.0 50.0 58.7 58.0 310-365 bp 1.0 80.1
psbI-trnS-GCU_p3 GCGTAATCCTGGACGTGAAG TTTTTCGTACCGAGGGTTCG 55.0 50.0 58.7 58.2 272-327 bp 1.0 79.2
psbI-trnS-GCU_p4 CGTAATCCTGGACGTGAAGA TGGACTAAAGCGTCGGATTG 50.0 50.0 57.1 58.0 308-363 bp 1.0 78.4
psbI-trnS-GCU_p5 CGTAATCCTGGACGTGAAGA GTGGACTAAAGCGTCGGATT 50.0 50.0 57.1 58.0 309-364 bp 1.0 78.4