Hotspot

trnK-UUU-rps16

LSC · 4427-5564 · 1138 bp

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Pi 0.0153
MarkerSeek score 62.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1138
variable_sites
45
pi
0.0153
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCGGTCTTACAAACTC ATCCCAATGGGCCGTTTATC 50.0 50.0 57.9 58.0 1490-1495 bp 1.0 86.0
trnK-UUU-rps16_p2 GTCGCGGTCTTACAAACTCT ATCCCAATGGGCCGTTTATC 50.0 50.0 57.9 58.0 1489-1494 bp 1.0 86.0
trnK-UUU-rps16_p3 AACCTTGGGGCTCTATCCAT ATCCCAATGGGCCGTTTATC 50.0 50.0 58.1 58.0 1711-1738 bp 1.0 85.9
trnK-UUU-rps16_p4 ACCTTGGGGCTCTATCCATT ATCCCAATGGGCCGTTTATC 50.0 50.0 58.1 58.0 1710-1737 bp 1.0 85.9
trnK-UUU-rps16_p5 GTCGCGGTCTTACAAACTCT TTTTCTTGAGCCGTACGAGG 50.0 50.0 57.9 57.9 1552-1559 bp 1.0 85.5