Hotspot

rpoA

LSC · 82216-83238 · 1023 bp

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Pi 0.00067
MarkerSeek score 43.20
Primer available yes
Label rpoA

Feature Metadata

label_name
rpoA
feature_type
gene
region
LSC
length_bp
1023
variable_sites
3
pi
0.00067
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoA_p1 TGCTAAAGATTCAAAGCCCCT GATGTAACCCCTATGCCACA 42.9 50.0 57.5 57.0 1217-1218 bp 0.933333 57.7
rpoA_p2 TGCTAAAGATTCAAAGCCCCT GTGATGTAACCCCTATGCCA 42.9 50.0 57.5 57.0 1219-1220 bp 0.933333 57.7
rpoA_p3 TGCTAAAGATTCAAAGCCCCT TGATGTAACCCCTATGCCAC 42.9 50.0 57.5 57.0 1218-1219 bp 0.933333 57.7
rpoA_p4 TGCTAAAGATTCAAAGCCCCT GTGATGTAACCCCTATGCCAC 42.9 52.4 57.5 58.4 1219-1220 bp 0.933333 54.5
rpoA_p5 TGCTAAAGATTCAAAGCCCCT GATGTAACCCCTATGCCACAT 42.9 47.6 57.5 57.5 1217-1218 bp 0.933333 53.8