Hotspot

cemA

LSC · 64548-65237 · 690 bp

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Pi 0.000386
MarkerSeek score 41.80
Primer available yes
Label cemA

Feature Metadata

label_name
cemA
feature_type
gene
region
LSC
length_bp
690
variable_sites
2
pi
0.000386
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
cemA_p1 AATTCGACGAATGAGGTGGG AAATCCTCGTCTCGGGAGAA 50.0 50.0 58.0 58.2 822 bp 1.0 78.8
cemA_p2 AATTCGACGAATGAGGTGGG AATCCTCGTCTCGGGAGAAA 50.0 50.0 58.0 58.2 821 bp 1.0 78.8
cemA_p3 AATTCGACGAATGAGGTGGG AGAAATCCTCGTCTCGGGAG 50.0 55.0 58.0 58.6 824 bp 1.0 75.2
cemA_p4 AATTCGACGAATGAGGTGGG AGGAGAAATCCTCGTCTCGG 50.0 55.0 58.0 58.6 827 bp 1.0 75.2
cemA_p5 AATTCGACGAATGAGGTGGG ATCCTCGTCTCGGGAGAAAG 50.0 55.0 58.0 58.6 820 bp 1.0 75.2