Hotspot

atpI

LSC · 15733-16476 · 744 bp

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Pi 0.000179
MarkerSeek score 41.50
Primer available yes
Label atpI

Feature Metadata

label_name
atpI
feature_type
gene
region
LSC
length_bp
744
variable_sites
1
pi
0.000179
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI_p1 TGGATTATCTTGGCCTAAGCT AGTGGAAAAGGAGATGGTTGA 42.9 42.9 56.5 56.8 855 bp 1.0 42.5
atpI_p2 TGGATTATCTTGGCCTAAGCT TCAAGTGGAAAAGGAGATGGT 42.9 42.9 56.5 56.8 858 bp 1.0 42.5
atpI_p3 TGGATTATCTTGGCCTAAGCT AAGTGGAAAAGGAGATGGTTGA 42.9 40.9 56.5 57.5 856 bp 1.0 40.2
atpI_p4 TGGATTATCTTGGCCTAAGCT AGTGGAAAAGGAGATGGTTGAA 42.9 40.9 56.5 57.5 855 bp 1.0 40.2
atpI_p5 TGGATTATCTTGGCCTAAGCT TCAAGTGGAAAAGGAGATGGTT 42.9 40.9 56.5 57.5 858 bp 1.0 40.2