Hotspot

ndhG-ndhI

SSC · 121472-121882 · 411 bp

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Pi 0.00204
MarkerSeek score 37.60
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
411
variable_sites
2
pi
0.00204
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
37.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 TCCCAACGAAAAGGCAGAAA TCGGCCTATGATCGTCATGA 45.0 50.0 57.9 58.4 648 bp 1.0 76.7
ndhG-ndhI_p2 TCCCAACGAAAAGGCAGAAA CGGCCTATGATCGTCATGAA 45.0 50.0 57.9 57.6 647 bp 1.0 76.3
ndhG-ndhI_p3 TCCCAACGAAAAGGCAGAAA TTCGGCCTATGATCGTCATG 45.0 50.0 57.9 57.6 649 bp 1.0 76.3
ndhG-ndhI_p4 TCCCAACGAAAAGGCAGAAA CTTTCGGCCTATGATCGTCA 45.0 50.0 57.9 57.5 651 bp 1.0 75.7
ndhG-ndhI_p5 ATTGGTCCAGGCAAATCCAT TCGGCCTATGATCGTCATGA 45.0 50.0 57.4 58.4 548 bp 1.0 72.8