Hotspot

trnP-UGG-psaJ

LSC · 70310-70796 · 487 bp

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Pi 0.00141
MarkerSeek score 41.50
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
487
variable_sites
3
pi
0.00141
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC CGCATCCGGGAATAATCGAT 50.0 50.0 58.1 58.0 650-651 bp 1.0 79.5
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA CGCATCCGGGAATAATCGAT 50.0 50.0 58.1 58.0 649-650 bp 1.0 79.5
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC CGCATCCGGGAATAATCGAT 50.0 50.0 58.1 58.0 661-662 bp 1.0 79.4
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA CGCATCCGGGAATAATCGAT 50.0 50.0 58.1 58.0 660-661 bp 1.0 79.4
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG CGCATCCGGGAATAATCGAT 50.0 50.0 58.1 58.0 663-664 bp 1.0 79.4