Hotspot

psaA-ycf3

LSC · 44151-44855 · 705 bp

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Pi 0.00124
MarkerSeek score 42.50
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
705
variable_sites
2
pi
0.00124
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 TGAGAAATGACCCGGTTTGG ATCAAGCCGCTGAGTATTGG 50.0 50.0 58.1 58.0 900-903 bp 1.0 79.3
psaA-ycf3_p2 GCCCATTCCTCGAAAGAAGT ATCAAGCCGCTGAGTATTGG 50.0 50.0 57.9 58.0 881-884 bp 1.0 79.1
psaA-ycf3_p3 TGAGAAATGACCCGGTTTGG CGATCAAGCCGCTGAGTATT 50.0 50.0 58.1 58.1 902-905 bp 1.0 78.5
psaA-ycf3_p4 GCCCATTCCTCGAAAGAAGT CGATCAAGCCGCTGAGTATT 50.0 50.0 57.9 58.1 883-886 bp 1.0 78.3
psaA-ycf3_p5 TGAGAAATGACCCGGTTTGG AGCCGCTGAGTATTGGAAAC 50.0 50.0 58.1 58.3 896-899 bp 1.0 77.5