Hotspot

rpl14

LSC · 77121-77492 · 372 bp

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Pi 0
MarkerSeek score 24.40
Primer available yes
Label rpl14

Feature Metadata

label_name
rpl14
feature_type
gene
region
LSC
length_bp
372
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
24.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14_p1 AGCATATACGTAAAACACAATCT TCTTTCATTCCTTTGCAGTGA 30.4 38.1 54.2 55.7 480 bp 1.0 40.0
rpl14_p2 TCAACATGTTTTCCTTTTTCT TCTTTCATTCCTTTGCAGTGA 28.6 38.1 52.0 55.7 519 bp 1.0 40.0
rpl14_p3 AGCATATACGTAAAACACAATCT TCTTTCATTCCTTTGCAGTGAA 30.4 36.4 54.2 56.5 480 bp 1.0 40.0
rpl14_p4 AAGCATATACGTAAAACACAATCT TCTTTCATTCCTTTGCAGTGA 29.2 38.1 55.0 55.7 481 bp 1.0 40.0
rpl14_p5 TCAACATGTTTTCCTTTTTCTT TCTTTCATTCCTTTGCAGTGA 27.3 38.1 52.9 55.7 519 bp 1.0 40.0