Hotspot

rpl32-trnL-UAG

SSC · 104521-105043 · 523 bp

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Pi 0.0127
MarkerSeek score 50.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
523
variable_sites
10
pi
0.0127
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CAACGAGCATCCAAAACCAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.2 57.9 599 bp 1.0 74.0
rpl32-trnL-UAG_p2 CAACGAGCATCCAAAACCAA AGCAGCGTGTCTACCAATTT 45.0 45.0 57.2 57.8 592 bp 1.0 73.5
rpl32-trnL-UAG_p3 CAACGAGCATCCAAAACCAA GCTTGAGCACTGCTTCCTAA 45.0 50.0 57.2 58.2 613 bp 1.0 73.5
rpl32-trnL-UAG_p4 CAACGAGCATCCAAAACCAA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.2 57.8 598 bp 1.0 73.4
rpl32-trnL-UAG_p5 CAACGAGCATCCAAAACCAA CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.2 57.8 597 bp 1.0 73.4