Hotspot

ndhF-rpl32

SSC · 103624-104355 · 732 bp

Back to Tragus

Pi 0.00274
MarkerSeek score 60.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
732
variable_sites
3
pi
0.00274
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGGAGAAGAGGA TGACATCGAAGTACGTTTTT 55.0 35.0 58.1 53.2 815-817 bp 1.0 41.9
ndhF-rpl32_p2 CTGGAAGTGGGAGAAGAGGA TTGACATCGAAGTACGTTTT 55.0 35.0 58.1 53.2 816-818 bp 1.0 41.9
ndhF-rpl32_p3 CTGGAAGTGGGAGAAGAGGA TTTGACATCGAAGTACGTTT 55.0 35.0 58.1 53.2 817-819 bp 1.0 41.9
ndhF-rpl32_p4 CTGGAAGTGGGAGAAGAGGA TTTTGACATCGAAGTACGTT 55.0 35.0 58.1 53.2 818-820 bp 1.0 41.9
ndhF-rpl32_p5 CTGGAAGTGGGAGAAGAGGA TTTTTGACATCGAAGTACGT 55.0 35.0 58.1 53.2 819-821 bp 1.0 41.9