Hotspot

petA-psbJ

LSC · 60625-61536 · 912 bp

Back to Tragus

Pi 0.00512
MarkerSeek score 49.50
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
912
variable_sites
7
pi
0.00512
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CGTCCAAGGCCTTTTGTTCT TGGTTCGTATTCTGGATTGGG 50.0 47.6 58.7 57.7 1055-1056 bp 1.0 64.9
petA-psbJ_p2 CGTCCAAGGCCTTTTGTTCT GGTTCGTATTCTGGATTGGGT 50.0 47.6 58.7 57.7 1054-1055 bp 1.0 64.9
petA-psbJ_p3 CGTCCAAGGCCTTTTGTTCT GCTGATAGGTACTGTAACCGG 50.0 52.4 58.7 57.6 1112-1113 bp 1.0 64.1
petA-psbJ_p4 CGTCCAAGGCCTTTTGTTCT GGCTGATAGGTACTGTAACCG 50.0 52.4 58.7 57.6 1113-1114 bp 1.0 64.1
petA-psbJ_p5 GATCCATTACGCGTCCAAGG TGGTTCGTATTCTGGATTGGG 55.0 47.6 58.8 57.7 1066-1067 bp 1.0 64.1