Hotspot

trnR-UCU-rps14

LSC · 36901-37287 · 387 bp

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Pi 0.00346
MarkerSeek score 57.00
Primer available yes
Label trnR-UCU-rps14

Feature Metadata

label_name
trnR-UCU-rps14
feature_type
igs
region
LSC
length_bp
387
variable_sites
2
pi
0.00346
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-rps14_p1 AGAAGACCTCTGTCCTATCCA ATGCATGTTTGTTACCGGGT 47.6 45.0 56.9 58.1 462-464 bp 1.0 63.6
trnR-UCU-rps14_p2 AGAAGACCTCTGTCCTATCCA TCCGGACACATACTTCGAGA 47.6 50.0 56.9 57.9 490-492 bp 1.0 63.3
trnR-UCU-rps14_p3 AGGTTTAGAAGACCTCTGTCCT ATGCATGTTTGTTACCGGGT 45.5 45.0 57.9 58.1 468-470 bp 1.0 62.9
trnR-UCU-rps14_p4 AGAAGACCTCTGTCCTATCCA TTACCGGGTGCAACAAGATC 47.6 50.0 56.9 58.2 451-453 bp 1.0 62.8
trnR-UCU-rps14_p5 AGGTTTAGAAGACCTCTGTCCT TCCGGACACATACTTCGAGA 45.5 50.0 57.9 57.9 496-498 bp 1.0 62.6