Hotspot

trnC-GCA-rpoB

LSC · 19116-20126 · 1011 bp

Back to Tragus

Pi 0.00264
MarkerSeek score 60.40
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1011
variable_sites
4
pi
0.00264
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 AAAGGATTTGCAGTCCCCTG CCGGGTATTGTGGACATTCC 50.0 55.0 58.1 58.3 1095 bp 1.0 77.1
trnC-GCA-rpoB_p2 AAAGGATTTGCAGTCCCCTG CGGGTATTGTGGACATTCCC 50.0 55.0 58.1 58.3 1094 bp 1.0 77.1
trnC-GCA-rpoB_p3 AAAGGATTTGCAGTCCCCTG AAGTTCTTCTGCCAAGCCTT 50.0 45.0 58.1 57.6 1159 bp 1.0 76.7
trnC-GCA-rpoB_p4 AAAGGATTTGCAGTCCCCTG AGTTCTTCTGCCAAGCCTTG 50.0 50.0 58.1 58.4 1158 bp 1.0 76.6
trnC-GCA-rpoB_p5 AAAGGATTTGCAGTCCCCTG CAAGTTCTTCTGCCAAGCCT 50.0 50.0 58.1 58.4 1160 bp 1.0 76.6