Hotspot

matK-trnK-UUU

LSC · 3226-3899 · 674 bp

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Pi 0.00198
MarkerSeek score 60.30
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
674
variable_sites
2
pi
0.00198
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 ACATAATCATGGGCAAATGCA GCCGGTGTAATTCCATCGAT 38.1 50.0 56.5 58.1 874-876 bp 1.0 59.6
matK-trnK-UUU_p2 ACATAATCATGGGCAAATGCA CAATGTTTTGTCAGGAGCGG 38.1 50.0 56.5 57.7 893-895 bp 1.0 57.9
matK-trnK-UUU_p3 ATACCCTTCGAATTTTGCCA GCCGGTGTAATTCCATCGAT 40.0 50.0 55.3 58.1 788-790 bp 1.0 57.9
matK-trnK-UUU_p4 TACCCTTCGAATTTTGCCAT GCCGGTGTAATTCCATCGAT 40.0 50.0 55.3 58.1 787-789 bp 1.0 57.9
matK-trnK-UUU_p5 GAATACCCTTCGAATTTTGCCA GCCGGTGTAATTCCATCGAT 40.9 50.0 57.3 58.1 790-792 bp 1.0 57.7