Hotspot

clpP

LSC · 69931-71945 · 2015 bp

Back to Tragopogon

Pi 0

MarkerSeek score 57.70

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2015
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CGAATTAACGCGAAATCCGG	GGCTTTGTTACGTTTCCACA	50.0	45.0	58.0	56.9	2097-2098 bp	1.0	70.9
clpP_p2	CTCGGCAGGGGTAATTTCAA	GGCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2121-2122 bp	1.0	69.9
clpP_p3	AACTCGGCAGGGGTAATTTC	GGCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2123-2124 bp	1.0	69.9
clpP_p4	GAAACTCGGCAGGGGTAATT	GGCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2125-2126 bp	1.0	69.9
clpP_p5	TATGAAACTCGGCAGGGGTA	GGCTTTGTTACGTTTCCACA	50.0	45.0	58.1	56.9	2128-2129 bp	1.0	69.9