Hotspot

ccsA

SSC · 113931-114899 · 969 bp

Back to Tragopogon

Pi 0

MarkerSeek score 36.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TTCTTAATTAATTTAGGGGATTCC	ACACTACTTGATTCACGCGG	29.2	50.0	52.5	58.3	1116 bp	1.0	40.0
ccsA_p2	TTCTTAATTAATTTAGGGGATTCC	CACTACTTGATTCACGCGGT	29.2	50.0	52.5	58.3	1115 bp	1.0	40.0
ccsA_p3	TTCTTAATTAATTTAGGGGATTCC	ACTACTTGATTCACGCGGTT	29.2	45.0	52.5	57.5	1114 bp	1.0	40.0
ccsA_p4	TTTCTTAATTAATTTAGGGGATTCC	ACACTACTTGATTCACGCGG	28.0	50.0	53.3	58.3	1117 bp	1.0	40.0
ccsA_p5	TTTCTTAATTAATTTAGGGGATTCC	CACTACTTGATTCACGCGGT	28.0	50.0	53.3	58.3	1116 bp	1.0	40.0