Hotspot

ndhB-trnL-CAA

IRa · 142720-143298 · 579 bp

Back to Tragopogon

Pi 0.00345

MarkerSeek score 60.30

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRa
length_bp: 579
variable_sites: 2
pi: 0.00345
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	639 bp	1.0	79.7
ndhB-trnL-CAA_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	637 bp	1.0	79.7
ndhB-trnL-CAA_p3	TGCAATTGCTCAGGATACCC	TACCATTTCACCACCAAGGC	50.0	50.0	57.9	58.1	627 bp	1.0	79.1
ndhB-trnL-CAA_p4	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	634 bp	1.0	78.2
ndhB-trnL-CAA_p5	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	50.0	50.0	57.9	57.7	638 bp	1.0	77.6