Hotspot

ndhF-rpl32

SSC · 111652-112643 · 992 bp

Back to Tragopogon

Pi 0.00101

MarkerSeek score 59.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 992
variable_sites: 1
pi: 0.00101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	TTTAAGGCGGCCCAATATCC	50.0	50.0	58.0	58.0	1179 bp	1.0	79.7
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	TTTAAGGCGGCCCAATATCC	45.0	50.0	58.0	58.0	1177 bp	1.0	79.6
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	TTTAAGGCGGCCCAATATCC	45.0	50.0	58.0	58.0	1178 bp	1.0	79.6
ndhF-rpl32_p4	CTTTTTGTTGCCGTCGGAAA	TTTAAGGCGGCCCAATATCC	45.0	50.0	57.8	58.0	1185 bp	1.0	78.4
ndhF-rpl32_p5	TTTGTTGCCGTCGGAAAAAG	TTTAAGGCGGCCCAATATCC	45.0	50.0	57.8	58.0	1182 bp	1.0	78.4