Hotspot

trnL-CAA-ndhB

IRb · 93959-94537 · 579 bp

Back to Tragopogon

Pi 0.00345

MarkerSeek score 60.30

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 579
variable_sites: 2
pi: 0.00345
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	639 bp	1.0	79.7
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	637 bp	1.0	79.7
trnL-CAA-ndhB_p3	TACCATTTCACCACCAAGGC	TGCAATTGCTCAGGATACCC	50.0	50.0	58.1	57.9	627 bp	1.0	79.1
trnL-CAA-ndhB_p4	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.2	57.9	634 bp	1.0	78.2
trnL-CAA-ndhB_p5	TCTCGCGTGTCTACCATTTC	TGCAATTGCTCAGGATACCC	50.0	50.0	57.7	57.9	638 bp	1.0	77.6