Hotspot

atpB-rbcL

LSC · 54367-55171 · 805 bp

Back to Tragopogon

Pi 0

MarkerSeek score 59.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 805
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATACGCCCCAGTTTCGTTTT	CACCAGCTTTGAATCCAACAC	45.0	47.6	58.1	58.0	920-922 bp	1.0	71.2
atpB-rbcL_p2	GCCTGGCGGAAAAGCTATAT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	966-968 bp	1.0	71.2
atpB-rbcL_p3	ATACGCCCCAGTTTCGTTTT	ACCAGCTTTGAATCCAACACT	45.0	42.9	58.1	58.1	919-921 bp	1.0	71.0
atpB-rbcL_p4	GCCTGGCGGAAAAGCTATAT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.1	58.1	965-967 bp	1.0	70.9
atpB-rbcL_p5	ATACGCCCCAGTTTCGTTTT	ACACCAGCTTTGAATCCAACA	45.0	42.9	58.1	58.3	921-923 bp	1.0	68.7