Hotspot

trnM-CAU-atpE

LSC · 52258-52471 · 214 bp

Back to Tragopogon

Pi 0

MarkerSeek score 55.20

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 214
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	AGCGGAAACTGCTTTGAGAA	50.0	45.0	58.4	58.0	366-372 bp	1.0	77.7
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	CGGGTAGAGGCTATCAATGC	50.0	55.0	58.4	57.9	287-293 bp	1.0	77.7
trnM-CAU-atpE_p3	TATTGCTTTCATACGGCGGG	CAAATCTAGCTCTCCGACGG	50.0	55.0	58.4	57.9	316-322 bp	1.0	77.2
trnM-CAU-atpE_p4	TATTGCTTTCATACGGCGGG	GGCAAATCTAGCTCTCCGAC	50.0	55.0	58.4	58.1	318-324 bp	1.0	77.1
trnM-CAU-atpE_p5	TATTGCTTTCATACGGCGGG	GAGGCAAATCTAGCTCTCCG	50.0	55.0	58.4	57.9	320-326 bp	1.0	77.0