Hotspot

psbM-trnD-GUC

LSC · 10868-11523 · 656 bp

Back to Tragopogon

Pi 0.00152

MarkerSeek score 59.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 656
variable_sites: 1
pi: 0.00152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.6	58.3	773 bp	1.0	50.5
psbM-trnD-GUC_p2	AAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	40.0	50.0	54.5	58.3	767 bp	1.0	50.1
psbM-trnD-GUC_p3	GGAACTAAAATGAACAGTGCAGT	TCCCCGGGATTGTAGTTCAA	39.1	50.0	57.6	58.3	788 bp	1.0	49.9
psbM-trnD-GUC_p4	GGAACTAAAATGAACAGTGCAGT	TTCCCCGGGATTGTAGTTCA	39.1	50.0	57.6	58.3	789 bp	1.0	49.9
psbM-trnD-GUC_p5	AAAATGAACAGTGCAGTAGC	TCCCCGGGATTGTAGTTCAA	40.0	50.0	54.5	58.3	782 bp	1.0	49.4