Hotspot

trnS-GGA-trnC-GCA

LSC · 8622-9358 · 737 bp

Back to Tragopogon

Pi 0.00136

MarkerSeek score 59.50

Primer available yes

Label trnS-GGA-trnC-GCA

Feature Metadata

label_name: trnS-GGA-trnC-GCA
feature_type: igs
region: LSC
length_bp: 737
variable_sites: 1
pi: 0.00136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-trnC-GCA_p1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	888 bp	1.0	78.2
trnS-GGA-trnC-GCA_p2	ACGAAAACGGAAAGAGAGGG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	894 bp	1.0	78.1
trnS-GGA-trnC-GCA_p3	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	870 bp	1.0	76.0
trnS-GGA-trnC-GCA_p4	ACGAAAACGGAAAGAGAGGG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.8	58.4	876 bp	1.0	75.9
trnS-GGA-trnC-GCA_p5	CGAAAACGGAAAGAGAGGGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	893 bp	1.0	75.8