Hotspot

psbA-trnK-UUU

LSC · 1552-1805 · 254 bp

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Pi 0
MarkerSeek score 56.30
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
254
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 TAAAGACGGTTTTCGGTGCT GAAAGCCGTGTGCAATGAAA 45.0 45.0 57.8 57.9 446-456 bp 1.0 78.8
psbA-trnK-UUU_p2 TAAAGACGGTTTTCGGTGCT CTAGTTCCGGGTTCGAATCC 45.0 55.0 57.8 57.8 361-371 bp 1.0 78.5
psbA-trnK-UUU_p3 GTAAAGACGGTTTTCGGTGC GAAAGCCGTGTGCAATGAAA 50.0 45.0 57.7 57.9 447-457 bp 1.0 78.3
psbA-trnK-UUU_p4 GTAAAGACGGTTTTCGGTGC CTAGTTCCGGGTTCGAATCC 50.0 55.0 57.7 57.8 362-372 bp 1.0 78.0
psbA-trnK-UUU_p5 TTTTCGGTGCTGGTTATCCA GAAAGCCGTGTGCAATGAAA 45.0 45.0 57.4 57.9 437-447 bp 1.0 76.2