Hotspot

rps15-ycf1

SSC · 131653-132357 · 705 bp

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Pi 0.00719

MarkerSeek score 64.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 705
variable_sites: 5
pi: 0.00719
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AGAACCCTGGTTTTCTTCTT	AATGGCAGCCGTTTCAGTAT	40.0	45.0	54.3	57.9	828-864 bp	1.0	51.2
rps15-ycf1_p2	AGAACCCTGGTTTTCTTCTT	ATAATGGCAGCCGTTTCAGT	40.0	45.0	54.3	57.9	830-866 bp	1.0	51.2
rps15-ycf1_p3	AGAACCCTGGTTTTCTTCTTT	AATGGCAGCCGTTTCAGTAT	38.1	45.0	55.2	57.9	828-864 bp	1.0	50.1
rps15-ycf1_p4	AGAACCCTGGTTTTCTTCTTT	ATAATGGCAGCCGTTTCAGT	38.1	45.0	55.2	57.9	830-866 bp	1.0	50.1
rps15-ycf1_p5	ACCCTGGTTTTCTTCTTTTTCT	AATGGCAGCCGTTTCAGTAT	36.4	45.0	56.0	57.9	825-861 bp	1.0	48.4