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rps3-rpl22

LSC · 90307-90905 · 599 bp

Back to Tradescantia

Pi 0.00673

MarkerSeek score 62.30

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 599
variable_sites: 4
pi: 0.00673
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	CCTCTAGACCCGCAGAGTAA	CGAGCTCGAGGACGTAGTTA	55.0	55.0	57.9	58.7	783-786 bp	1.0	74.8
rps3-rpl22_p2	ACCAAGTCTGAAACCGAGTG	CGAGCTCGAGGACGTAGTTA	50.0	55.0	57.8	58.7	719-722 bp	1.0	73.4
rps3-rpl22_p3	ATCTTCCTCTAGACCCGCAG	CGAGCTCGAGGACGTAGTTA	55.0	55.0	58.3	58.7	788-791 bp	1.0	72.8
rps3-rpl22_p4	TCATCTTCCTCTAGACCCGC	CGAGCTCGAGGACGTAGTTA	55.0	55.0	58.3	58.7	790-793 bp	1.0	72.7
rps3-rpl22_p5	CCAAGTCTGAAACCGAGTGG	CGAGCTCGAGGACGTAGTTA	55.0	55.0	58.5	58.7	718-721 bp	1.0	71.4