Hotspot

rpl20-rps12

LSC · 74527-75496 · 970 bp

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Pi 0

MarkerSeek score 60.30

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 970
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TAAGTCTCGAATGAGCCCCT	TCTCCCGCTCTTAGAGGATG	50.0	55.0	57.8	58.0	1102-1118 bp	1.0	79.8
rpl20-rps12_p2	GTAAGTCTCGAATGAGCCCC	TCTCCCGCTCTTAGAGGATG	55.0	55.0	57.8	58.0	1103-1119 bp	1.0	79.2
rpl20-rps12_p3	TAAGTCTCGAATGAGCCCCT	GTCACAAAATCTCCCGCTCT	50.0	50.0	57.8	57.9	1111-1127 bp	1.0	79.1
rpl20-rps12_p4	GTCTCGAATGAGCCCCTCTA	TCTCCCGCTCTTAGAGGATG	55.0	55.0	58.3	58.0	1099-1115 bp	1.0	78.6
rpl20-rps12_p5	GTAAGTCTCGAATGAGCCCC	GTCACAAAATCTCCCGCTCT	55.0	50.0	57.8	57.9	1112-1128 bp	1.0	78.5