Hotspot

petA-psbJ

LSC · 68034-68913 · 880 bp

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Pi 0.0114

MarkerSeek score 65.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 880
variable_sites: 10
pi: 0.0114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TTTGCGTCCGTTATTTTGGC	GGTTCATATTCCGGGTTGGG	45.0	55.0	57.9	58.3	999-1000 bp	1.0	78.0
petA-psbJ_p2	TTTGCGTCCGTTATTTTGGC	CATATTCCGGGTTGGGTTCA	45.0	50.0	57.9	57.6	995-996 bp	1.0	77.1
petA-psbJ_p3	TTTGCGTCCGTTATTTTGGC	GTTCATATTCCGGGTTGGGT	45.0	50.0	57.9	57.6	998-999 bp	1.0	77.1
petA-psbJ_p4	TTTGCGTCCGTTATTTTGGC	CGGTTCATATTCCGGGTTGG	45.0	55.0	57.9	58.7	1000-1001 bp	1.0	75.0
petA-psbJ_p5	TTTGCGTCCGTTATTTTGGC	TATTCCGGGTTGGGTTCATC	45.0	50.0	57.9	57.3	993-994 bp	1.0	74.8