Hotspot

accD-psaI

LSC · 64083-64682 · 600 bp

Back to Tradescantia

Pi 0.00833

MarkerSeek score 63.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 600
variable_sites: 5
pi: 0.00833
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCGACCAAATTGTACCACGT	AAAGAAGCCATCGCAATTGC	45.0	45.0	57.5	58.0	775-1396 bp	1.0	76.1
accD-psaI_p2	AAAAGGTGTTCTGGCTGAGT	AAAGAAGCCATCGCAATTGC	45.0	45.0	57.3	58.0	747-1368 bp	1.0	74.5
accD-psaI_p3	AAAGGTGTTCTGGCTGAGTT	AAAGAAGCCATCGCAATTGC	45.0	45.0	57.3	58.0	746-1367 bp	1.0	74.5
accD-psaI_p4	TCGACCAAATTGTACCACGT	CCGGGAATACTAGGCCTACT	45.0	55.0	57.5	57.7	756-1377 bp	1.0	73.7
accD-psaI_p5	TCGACCAAATTGTACCACGT	CGCAATTGCCGGGAATACTA	45.0	50.0	57.5	58.4	764-1385 bp	1.0	72.8