Hotspot

trnT-GGU-psbD

LSC · 36167-37136 · 970 bp

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Pi 0.00106
MarkerSeek score 61.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
970
variable_sites
1
pi
0.00106
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG CTACCAATGGCTGCAGTCAT 55.0 50.0 58.1 58.0 1025-1048 bp 1.0 81.0
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA CTACCAATGGCTGCAGTCAT 50.0 50.0 58.1 58.0 1028-1051 bp 1.0 80.8
trnT-GGU-psbD_p3 CGTAAGTCGTCGGTTCGAAT CTACCAATGGCTGCAGTCAT 50.0 50.0 58.1 58.0 1000-1023 bp 1.0 80.6
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG AAGTCTACCAATGGCTGCAG 55.0 50.0 58.1 57.9 1029-1052 bp 1.0 80.4
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA AAGTCTACCAATGGCTGCAG 50.0 50.0 58.1 57.9 1032-1055 bp 1.0 80.2