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petN-psbM

LSC · 31978-33224 · 1247 bp

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Pi 0.00481

MarkerSeek score 61.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1247
variable_sites: 6
pi: 0.00481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	TGCTACTGCACTGTTTATCT	55.0	40.0	58.1	54.4	1351-1354 bp	1.0	50.6
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTTATCT	55.0	40.0	58.1	54.4	1350-1353 bp	1.0	50.6
petN-psbM_p3	TCCCTCTCGCTCGTAGTATG	TGCTACTGCACTGTTTATCT	55.0	40.0	58.1	54.4	1365-1368 bp	1.0	50.5
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTTATCT	50.0	40.0	58.2	54.4	1409-1412 bp	1.0	50.3
petN-psbM_p5	TATGGGGAAGGAGTGGACTC	TGCTACTGCACTGTTTATCT	55.0	40.0	57.8	54.4	1349-1352 bp	1.0	50.1