Hotspot

matK-trnK-UUU

LSC · 3285-4434 · 1150 bp

Back to Tradescantia

Pi 0.00441

MarkerSeek score 62.50

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1150
variable_sites: 5
pi: 0.00441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AGAAGATGCTGTTGCCAAGA	TCGGGGTTGCTAACTCAATG	45.0	50.0	57.4	57.9	1239-1246 bp	1.0	75.8
matK-trnK-UUU_p2	AGAAGATGCTGTTGCCAAGA	GGGGTTGCTAACTCAATGGT	45.0	50.0	57.4	57.8	1237-1244 bp	1.0	75.0
matK-trnK-UUU_p3	AGAAGATGCTGTTGCCAAGAT	TCGGGGTTGCTAACTCAATG	42.9	50.0	57.9	57.9	1239-1246 bp	1.0	71.8
matK-trnK-UUU_p4	AGAAGATGCTGTTGCCAAGAT	GGGGTTGCTAACTCAATGGT	42.9	50.0	57.9	57.8	1237-1244 bp	1.0	70.9
matK-trnK-UUU_p5	AGAAGATGCTGTTGCCAAGA	CGGGGTTGCTAACTCAATGG	45.0	55.0	57.4	58.9	1238-1245 bp	1.0	69.5