Hotspot

ycf4

LSC · 60196-60750 · 555 bp

Back to Trachyspermum

Pi 0.0042

MarkerSeek score 60.40

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 555
variable_sites: 3
pi: 0.0042
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	AGTTGATGAGAGTTACTTCGGA	GCATTCACTCTCTCAGCTCA	40.9	50.0	56.6	57.3	662 bp	1.0	47.8
ycf4_p2	TGATGAGAGTTACTTCGGAAACA	GCATTCACTCTCTCAGCTCA	39.1	50.0	57.5	57.3	659 bp	1.0	47.3
ycf4_p3	AGTTGATGAGAGTTACTTCGGA	AGCATTCACTCTCTCAGCTC	40.9	50.0	56.6	57.0	663 bp	1.0	45.4
ycf4_p4	AGTTGATGAGAGTTACTTCGGAA	GCATTCACTCTCTCAGCTCA	39.1	50.0	57.3	57.3	662 bp	1.0	45.2
ycf4_p5	TGATGAGAGTTACTTCGGAAACA	AGCATTCACTCTCTCAGCTC	39.1	50.0	57.5	57.0	660 bp	1.0	44.9