Hotspot

ycf1

SSC · 124064-129556 · 5493 bp

Back to Trachyspermum

Pi 0.00534

MarkerSeek score 43.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5493
variable_sites: 44
pi: 0.00534
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGACAAACGAATGGGATCGA	CCACAAAAATGGCGAACTACA	45.0	42.9	56.9	57.4	1197-1203 bp	1.0	60.0
ycf1_p2	AGACAAACGAATGGGATCGA	ACCACAAAAATGGCGAACTAC	45.0	42.9	56.9	57.4	1198-1204 bp	1.0	60.0
ycf1_p3	CCGCGGGTTCTTTTTCTTCT	CCACAAAAATGGCGAACTACA	50.0	42.9	58.8	57.4	856 bp	0.666667	48.0
ycf1_p4	CCGCGGGTTCTTTTTCTTCT	ACCACAAAAATGGCGAACTAC	50.0	42.9	58.8	57.4	857 bp	0.666667	48.0
ycf1_p5	CGCGGGTTCTTTTTCTTCTT	CCACAAAAATGGCGAACTACA	45.0	42.9	56.9	57.4	855 bp	0.666667	45.7