Hotspot

psbA

LSC · 289-1350 · 1062 bp

Back to Trachyspermum

Pi 0

MarkerSeek score 27.10

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TCTTGTATATACTAAAACCCGGTC	AAATTCGTGTGCTTGGGAGT	37.5	45.0	55.8	58.0	1137 bp	1.0	40.0
psbA_p2	TCTTGTATATACTAAAACCCGGTC	TGCTTGGGAGTCCCTGATAA	37.5	50.0	55.8	58.0	1128 bp	1.0	40.0
psbA_p3	TCTTGTATATACTAAAACCCGGT	AAATTCGTGTGCTTGGGAGT	34.8	45.0	54.6	58.0	1137 bp	1.0	40.0
psbA_p4	TCTTGTATATACTAAAACCCGGT	TGCTTGGGAGTCCCTGATAA	34.8	50.0	54.6	58.0	1128 bp	1.0	40.0
psbA_p5	CTTGTATATACTAAAACCCGGTC	AAATTCGTGTGCTTGGGAGT	39.1	45.0	54.4	58.0	1136 bp	1.0	40.0